• Skip the Organizational structure panel
• Organizational structure

  On selecting a constituent part of MU the "Overview of publishing activities" page will be displayed with information relevant to the selected constituent part. The "Overview of publishing activities" page is not available for non-activated items.

  Masaryk University

  • Faculties
  • Law
  • Medicine
  • Science
  • Arts
  • Education
  • Economics
  • Informatics
  • Social Studies
  • Sports Studies

  • Institutes
  • Computer Science
  • CEITEC MU
  • Politica
  • Specialized Units
  • Hostels & Canteens
  • University Press
  • Telč Centre
  • UCB Management

  • Rector`s Office
  • Rectorate
  • Other Units
  • Archive
  • Language Centre
  • International Studies
  • Disabled Students
  • Technology Transfer
  • Biostatistics
  • Mendel Museum
  • CERIT
  • Civic Education
  • Project Team CEITEC

   

Publication details

Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach

Basic information
Original title:	Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
Authors:	Pavel Bouchal, Iva Struhárová, Eva Budinská, Ondrej Šedo, Tereza Vyhlídalová, Zbyněk Zdráhal, Rob van Spanning, Igor Kučera

Further information
Citation:	BOUCHAL, Pavel - STRUHÁROVÁ, Iva - BUDINSKÁ, Eva - ŠEDO, Ondrej - VYHLÍDALOVÁ, Tereza - ZDRÁHAL, Zbyněk - VAN SPANNING, Rob - KUČERA, Igor. Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach. Biochimica et Biophysica Acta - Proteins and Proteomics, Amsterdam, The Netherlands, Elsevier, The Nederlands. ISSN 1570-9639, 2010, vol. 1804, no. 6, pp. 1309-1316.
Original language:	English
Field:	Biochemistry
WWW:	Odkaz na článek
Type:	Article in Periodical
Keywords:	Paracoccus denitrificans; FNR transcription regulator; Respiratory switch; Two-dimensional gel electrophoresis; Proteome

The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation.

Related projects:

• Proteins in metabolism and interaction of organisms with the environment
• Molecular basis of cell and tissue regulations
• The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques