Informace o publikaci
Immunosensors and immunoassays for detection of clinically important analytes
| Autoři | |
|---|---|
| Rok publikování | 2018 |
| Druh | Vyžádané přednášky |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Highly sensitive and specific bioanalysis is essential in all fields of human activities from food industry to clinical diagnostics. Among many analytical techniques, immunoassays and immunosensors are gaining attention due to the high specificity provided by antibodies and excellent sensitivity provided by various labels. We have employed immunosensors for the detection of Salmonella Typhimurium, which belongs to leading agents of gastrointestinal diseases. The point-of-care sensor was based on electrochemical impedance spectroscopy and screen-printed electrodes modified by specific antibody. We have further developed a signal enhancement by enzymatic precipitation for highly sensitive laboratory-based detection of Salmonella using surface plasmon resonance. Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals, which convert near-infrared light to light of shorter wavelengths. We have exploited UCNPs as a label in upconversion-linked immunoassay for the detection of pharmaceutical diclofenac, which is a common micropollutant in waters. The low background and high photostability make UCNPs a powerful tool for detection of single molecules. We have developed an optical approach for visualizing individual UCNPs and applied it for the sensitive detection of the cancer biomarker prostate specific antigen. The noise-surpassing digital readout provided single particle resolution with almost no instrumental background. |
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