Informace o publikaci

Memory CD4(+) T cells are generated in the human fetal intestine

Autoři	LI N. VAN UNEN V. ABDELAAL T. GUO N.N. KASATSKAYA S.A. LADELL K. MCLAREN J.E. EGOROV E.S. IZRAELSON M. LOPES S.M.C.D. HOLLT T. BRITANOVA O.V. EGGERMONT J. DE MIRANDA N.F.C.C. CHUDAKOV Dmitriy PRICE D.A. LELIEVELDT B.P.F. KONING F.
Rok publikování	2019
Druh	Článek v odborném periodiku
Časopis / Zdroj	Nature immunology
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://dx.doi.org/10.1038/s41590-018-0294-9
Doi	http://dx.doi.org/10.1038/s41590-018-0294-9
Klíčová slova	REPERTOIRE
Popis	The fetus is thought to be protected from exposure to foreign antigens, yet CD45RO(+) T cells reside in the fetal intestine. Here we combined functional assays with mass cytometry, single-cell RNA sequencing and high-throughput T cell antigen receptor (TCR) sequencing to characterize the CD4(+) T cell compartment in the human fetal intestine. We identified 22 CD4(+) T cell clusters, including naive-like, regulatory-like and memory-like subpopulations, which were confirmed and further characterized at the transcriptional level. Memory-like CD4(+) T cells had high expression of Ki-67, indicative of cell division, and CD5, a surrogate marker of TCR avidity, and produced the cytokines IFN-gamma and IL-2. Pathway analysis revealed a differentiation trajectory associated with cellular activation and proinflammatory effector functions, and TCR repertoire analysis indicated clonal expansions, distinct repertoire characteristics and interconnections between subpopulations of memory-like CD4(+) T cells. Imaging mass cytometry indicated that memory-like CD4(+) T cells colocalized with antigen-presenting cells. Collectively, these results provide evidence for the generation of memory-like CD4(+) T cells in the human fetal intestine that is consistent with exposure to foreign antigens.