Informace o projektu
Capillary electrophoresis as a member of the metabolomic analytical toolbox
(CE metabolomics)
- Kód projektu
- GAP206/11/0009
- Období řešení
- 1/2011 - 12/2015
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
- Přírodovědecká fakulta
- Další fakulta/pracoviště MU
- Lékařská fakulta
The generic methods for metabolomic studies based on application of different CE modes - capillary zone electrophoresis (CZE), micellar electrokinetic capillary chromatography (MEKC), microemulsion electrokinetic capillary chromatography (MEEKC), nonaqueous capillary electrophoresis (NACE) for corresponding metabolite classes will be developed. The relevant on-line preconcentration strategies will be integrated in given methods and possibility of using with MS detection will be tested. They can be used for metabolite fingerprinting and footprinting, metabolite profiling as well as target analysis. The complete procedure for the target metabolome analysis of microbial samples thus will be available starting from the rapid metabolism quenching and the quantitative extraction of selected metabolites up to their analysis by different CE modes. Besides the new method for the embryo viability testing based on non-invasive metabolite footprinting and profiling will be available as well.
Publikace
Počet publikací: 46
2014
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Capillary electrophoresis with contactless conductivity detection for amino acid profiling of human plasma samples
11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014, rok: 2014
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Capillary electrophoresis with contactless conductivity detection for the analysis of energetically important compounds for use in human assisted reproduction
Rok: 2014, druh: Konferenční abstrakty
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Determination of binding constants of selected sulfonylureas drugs
Rok: 2014, druh: Konferenční abstrakty
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Determination of metabolites of caffeine in saliva and urine as a measure of CYP1A2 metabolic activity
Acta Universitatis Palackianae Olomucensis Chemica 51S, rok: 2014
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Determination of Plasma Thiamine and Its Phosphorylated Esters - Study of Gestational Diabetes
Rok: 2014
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In-capillary derivatization of amino acid followed by capillary electrophoresis with fluorescence detection
Rok: 2014, druh: Konferenční abstrakty
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Metabolomics: the tool for the improvement of pregnancy outcome in the human assisted reproduction
Rok: 2014, druh: Konferenční abstrakty
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Method development for the analysis of carboxylic acids as a part of a metabolomic toolbox for a human embryo viability assessment
Rok: 2014, druh: Konferenční abstrakty
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Nano scale derivatization followed by capillary electrophoresis with fluorescence detection
Rok: 2014, druh: Konferenční abstrakty
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Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014, rok: 2014