Informace o publikaci
Efficient transfer of antibiotic resistance plasmids by transduction within methicillin-resistant Staphylococcus aureus USA300 clone
| Název česky | Účinný přenos plazmidů kódujících antibiotické rezistence transdukcí u meticilin-rezistentního klonu Staphylococcus aureus USA300 |
|---|---|
| Autoři | |
| Rok publikování | 2012 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | FEMS Microbiology Letters |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x |
| Doi | http://dx.doi.org/10.1111/j.1574-6968.2012.02589.x |
| Obor | Genetika a molekulární biologie |
| Klíčová slova | antimicrobial drug resistance; penicillinase plasmids; Staphylococcus bacteriophage; lysogeny; horizontal gene transfer; plasmid transduction |
| Přiložené soubory | |
| Popis | The epidemic community-associated methicillin-resistant clone Staphylococcus aureus USA300 is a major source of skin and soft tissue infections and involves strains with a diverse set of resistance genes. In this study, we report efficient transduction of penicillinase and tetracycline resistance plasmids by bacteriophages phi80alpha and phiJB between clinical isolates belonging to the USA300 clone. High transduction frequencies (10-5 to 10-6 CFU/PFU) were observed using phages propagated on donor strains as well as prophages induced from donors by ultraviolet light. Quantitative real-time PCR was employed to detect penicillinase plasmids in transducing phage particles and determine the ratio of transducing particles in phage lysates to infectious phage particles (determined as approximately 1:1700). Successful transfer of plasmids between strains in USA300 clone proves transduction is an effective mechanism for spreading plasmids within the clone. Such events contribute to its evolution and to emergence of new multiple drug-resistant strains of this successful clone. |
| Související projekty: |