Informace o publikaci

Comparing biocompatibility of gingival fibroblasts and bacterial strains on a different modified titanium discs

Autoři	FRANKOVA Jana PIVODOVÁ Veronika RŮŽIČKA Filip TOMÁNKOVÁ Kateřina ŠAFÁŘOVÁ Klára VRBKOVÁ Jana ULRICHOVÁ Jitka
Rok publikování	2013
Druh	Článek v odborném periodiku
Časopis / Zdroj	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
Doi	http://dx.doi.org/10.1002/jbm.a.34598
Obor	Mikrobiologie, virologie
Klíčová slova	titanium modification; bacterial strains; gingival fibroblasts; adhesion
Popis	The modification of implant surface situated in the area of peri-implant sulcus has important role in bacterial and cell adhesion. Six different chemically and physically modified titanium discs were prepared: glazed (Tis-MALP), unglazed (Tis-O), unglazed and alkali-etched (Tis-OA), unglazed and coated with ZrN (Tis-OZ), unglazed, sand blasted, and acid etched (Tis-OPAE), and unglazed, sand blasted, acid, and alkali etched (Tis-OPAAE). Analysis of surface topography was determined using scanning electron microscopy and atomic force microscopy (AFM). Biocompatibility of gingival fibroblasts was characterized by the production of tumor necrosis factor alpha, collagen I, matrix metalloproteinase 2 (MMP-2) after 24 and 72 h and expression of (31) integrin and vinculin using enzyme-linked immunosorbent assay (ELISA) or modified ELISA after 6 and 24 h. Microorganism adhesion (five bacterial strains) and biofilm formation was also evaluated. The adhesion of bacteria and gingival fibroblasts was significantly higher on titanium disc Tis-OPAAE and biofilm formation on the same surface for Streptococcus mutans, Streptococcus gordonii, and Streptococcus intermedius. The gingival fibroblasts on Tis-OPAAE disc had also significantly lower production of MMP-2. The collagen production was significantly lower on all surfaces with roughness higher than 0.2 m. This study confirmed that the titanium disc with the surface roughness 3.39 m (Tis-OPAAE) supported the adhesion of bacterial strains as well as gingival fibroblasts.