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Informace o publikaci
Electrochemical behaviour of 2,4-dinitrophenylhydrazi(o)ne asmulti-redox centre DNA label at mercury meniscus modified silversolid amalgam electrode
Autoři | |
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Rok publikování | 2014 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Electrochimica Acta |
Fakulta / Pracoviště MU | |
Citace | |
www | http://dx.doi.org/10.1016/j.electacta.2013.09.147 |
Doi | http://dx.doi.org/10.1016/j.electacta.2013.09.147 |
Obor | Elektrochemie |
Klíčová slova | DNA; Redox labeling; 2_4-Dinitrophenylhydrazine; Silver solid amalgam electrode; Voltammetry |
Popis | A detailed voltammetric study of 2,4-dinitrophenylhydrazine (DNPH), a DNPH modified nucleotide(dCDNPHMP) and a short single stranded DNA (ssDNA-DNPH) at mercury meniscus modified silver solid amalgam electrode (m-AgSAE) is presented in this work. Electrochemical behaviour of these compounds was studied using cyclic voltammetry (CV), adsorptive stripping CV and open circuit adsorptive transfer stripping CV. Successful incorpo-ration of the DNPH labeled nucleoside triphosphate into the DNA structure shows that selected DNApolymerase tolerates this bulky moiety and can be used, in combination with electrochemical methodsutilizing m-AgSAE, in further development of various applications. |
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