Informace o publikaci
Detection of p53 Gene by Using Genomagnetic Assay Combined with Carbon Nanotube Modified Disposable Sensor Technology
| Autoři | |
|---|---|
| Rok publikování | 2015 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Electroanalysis |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://onlinelibrary.wiley.com/doi/10.1002/elan.201400731/epdf |
| Doi | http://dx.doi.org/10.1002/elan.201400731 |
| Obor | Elektrochemie |
| Klíčová slova | p53 Gene; Carbon nanotubes; Magnetic particles; DNA hybridization; Differential pulse voltammetry |
| Přiložené soubory | |
| Popis | Electrochemical monitoring of DNA hybridization related to p53 gene sequence was investigated using genomagnetic assay combined with single walled carbon nanotube (SWCNT) modified pencil graphite electrodes (PGEs). The hybridization was performed either at magnetic beads (MB) surface or in solution. The enhanced guanine signal was obtained using SWCNT-PGEs compared to one obtained by unmodified PGEs. The selectivity of genomagnetic assay was tested under optimum conditions. The DLs were calculated as 0.88 mu M and 0.11 mu M for hybridization performed at MB surface and solution, respectively. This selective, practical and cost effective genomagnetic assay combined with SWCNT-PGEs is reported herein for the first time. |