Informace o publikaci

The crucial role of emilin 1 gene expression during progression of tumor growth

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RABAJDOVA Miroslava URBAN Peter SPAKOVA Ivana SAKSUN Ladislav DUDIC Rastislav OSTRO Alexander CAPRNDA Martin KRUŽLIAK Peter ADAMEK Mariusz MAREKOVA Maria

Rok publikování 2016
Druh Článek v odborném periodiku
Časopis / Zdroj Journal of cancer research and clinical oncology
Fakulta / Pracoviště MU

Lékařská fakulta

Citace
Doi http://dx.doi.org/10.1007/s00432-016-2226-0
Obor Onkologie a hematologie
Klíčová slova Breast cancer; Tumor microenvironment; Gene expression; Hypoxia; Metastases
Popis This study describes the effect of rapid tumor growth of patients suffering from various grades of malignant ductal breast carcinoma associated with the gene expression of ECM protein emilin 1, in correlation with the number of gene copies of emilin 1 and degradation of tumor tissue proteins. A total of 40 examined patients participated in the experiment (controls, n = 10, grades GI-GIII, each n = 10). After isolation of total mRNA, transcription of mRNA into the cDNA was performed. Quantification of gene expression changes was detected by the real-time PCR method. Analysis at the protein level was performed via Western blot method. During the detection of changes at the mRNA level, a significantly decreased level of emilin 1 in tumor tissues with grade II (about 54 +/- A 8 % lower than control) was identified. Protein-level analysis indicated an increased level of emilin 1 in tumors with grade I in comparison with control samples (about 10 +/- A 3 %). Obtained results demonstrated that the suppressive role of emilin 1 is related to the grade of growing breast tumors, and associated with increased hypoxia in the tumor microenvironment followed by elevated unfolding and degradation of tissue proteins.

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