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A scanning electron microscope technique for studying the sclerites of Cichlidogyrus

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FANNES Wouter VANHOVE Maarten Pieterjan HUYSE Tine PALADINI Giuseppe

Rok publikování 2015
Druh Článek v odborném periodiku
Časopis / Zdroj Parasitology Research
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.1007/s00436-015-4446-7
Obor Zoologie
Klíčová slova Platyhelminthes; Cichlidae; Anchor; MCO; Male apparatus
Popis The genus Cichlidogyrus (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. Cichlidogyrus has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual Cichlidogyrus worms. Twenty-year-old, formol-fixed specimens of Cichlidogyrus casuarinus were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections.
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