Informace o publikaci
Alveolar macrophages vs. Monocyte derived macrophages: different macrophages with different immune response to respiratory pathogens
| Autoři | |
|---|---|
| Rok publikování | 2016 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Alveolar macrophages as resident cells provide one of the first lines of defence against microbes invading the lung tissue. On the other hand, monocyte derived macrophages (MDMFs) as inflammatory macrophages are naive cells accumulating in site of inflammation. Macrophages serve as the target of replication of viruses like porcine reproductive and respiratory syndrome (PRRS) which is an important swine disease causing severe economic losses worldwide and can predispose pigs to infection by bacteria such as Haemophilus parasuis. Concurrent infection of porcine alveolar macrophages (PAMs) or MDMFs with PRRS virus and H. parasuis was analysed in vitro and difference dependent on macrophage type were observed. New infiltrated MDMFs were more sensitive to PRRSV infection resulting in higher mortality of cells and higher production of IFN-? compared to fully differentiated PAMs. Elevated level of IFN-? decreased expression of pro-inflammatory cytokines (IL-1ß and IL-8) which was confirmed by experimental addition of IFN-? to MDMFs followed by infection with H. parasuis. Furthermore, infection of MDMFs with H. parasuis caused cell fusions and formation of multinucleated giant cells. The work was supported by the Ministry of Education, Youth and Sports of the Czech Republic (project LO1218) and the Ministry of Agriculture of the Czech Republic (QJ1210120). |