Informace o publikaci
Universal Methods for Transgene Induction Using the Dexamethasone-Inducible Transcription Activation System pOp6/LhGR in Arabidopsis and Other Plant Species
| Autoři | |
|---|---|
| Rok publikování | 2019 |
| Druh | Článek v odborném periodiku |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://dx.doi.org/10.1002/cppb.20089 |
| Doi | http://dx.doi.org/10.1002/cppb.20089 |
| Klíčová slova | chemically inducible gene expression, glucocorticoid, dexamethasone |
| Přiložené soubory | |
| Popis | The use of chemically inducible systems for transgenes expression is a crucial requirement for modern plant biology research, as it allows (1) expression of transgenes that compromise plant viability or fertility when constitutively expressed and (2) spatio-temporal control of transgene expression levels. We describe the stringently regulated and highly responsive dexamethasone-inducible gene expression system pOp6/LhGR, which comprises of a chimeric transcription activator LhGR and a corresponding pOp6 promoter. Upon induction, the LhGR activator binds to the pOp6 promotor and induces expression of the target gene of interest. We provide detailed protocols for inducing transgene expression at different developmental stages and in different plant species and discuss dexamethasone stability and the use of its analogues. We also introduce new, versatile, GATEWAYTM compatible binary vectors that are now available for the pOp6/LhGR system. |