Informace o publikaci

Nanostructured Zirconium-Oxide Bioceramic Coatings Derived from the Anodized Al/Zr Metal Layers

Autoři	FOHLEROVÁ Zdenka KAMNEV Kirill SEPULVEDA Marcela PYTLICEK Zdenek PRASEK Jan MOZALEV Alexander
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	ADVANCED MATERIALS INTERFACES
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://onlinelibrary.wiley.com/doi/10.1002/admi.202100256
Doi	http://dx.doi.org/10.1002/admi.202100256
Klíčová slova	anodizing; bio-interfaces; biomaterials; nanostructures; porous anodic alumina; Saos-2 cells; ZrO2
Popis	Here, zirconium-oxide ceramic coatings comprising arrays of 3D nanostructures are electrochemically synthesized, ranging in shape, size, spacing, and population density, termed as the nanomounds (empty set approximate to 65 nm), nanopillars (empty set approximate to 130 nm), and nanostumps (empty set approximate to 220 nm). The nanostructured coatings, alongside a flat ZrO2 anodic film, are explored as a potential biomaterial in experiments with Saos-2 cells. All coatings reveal no cytotoxicity to living cells. The population density and spreading area of the cells, being the largest on the flat film, slightly decrease with increasing nanostructure dimensions. The cells progressively proliferate on all the surfaces, the nanomounds and, especially, nanopillars promoting the best viabilities and proliferation rates. The flat, nanomound, and nanopillar coatings promote the well-defined organizations of actin filaments across the entire cell bodies with no disruption in the cytoskeletal network and the mature large dash-shaped focal adhesions. The highest activity of alkaline phosphatase and the biggest deposition of a mineralization-competent extracellular matrix occur on the nanopillar array, the other nanostructures showing a better result than the flat coating, though. The differences, paradoxes, and regularities in the cell responses are explained through the detailed consideration of the coating morphologies, surface chemistries, and processes at the cell/surface interfaces.