Informace o publikaci

The genetic and epigenetic landscape of the Arabidopsis centromeres

Autoři	NAISH M. ALONGE M. WLODZIMIERZ P. TOCK A.J. ABRAMSON B.W. SCHMUCKER A. MALÍK MANDÁKOVÁ Terezie JAMGE B. LAMBING C. KUO P. YELINA N. HARTWICK N. COLT K. SMITH L.M. TON J. KAKUTANI T. MARTIENSSEN R.A. SCHNEEBERGER K. LYSÁK Martin BERGER F. BOUSIOS A. MICHAEL T.P. SCHATZ M.C. HENDERSON I.R.
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	Science
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	https://www.science.org/doi/10.1126/science.abi7489
Doi	http://dx.doi.org/10.1126/science.abi7489
Klíčová slova	HUMAN X-CHROMOSOME; TRANSPOSABLE ELEMENTS; SEQUENCE ORGANIZATION; READ ALIGNMENT; WILD-TYPE; GENOME; CHROMATIN; LOCALIZATION; SATELLITE; EVOLUTION
Popis	Centromeres attach chromosomes to spindle microtubules during cell division and, despite this conserved role, show paradoxically rapid evolution and are typified by complex repeats. We used long-read sequencing to generate the Col-CEN Arabidopsis thaliana genome assembly that resolves all five centromeres. The centromeres consist of megabase-scale tandemly repeated satellite arrays, which support CENTROMERE SPECIFIC HISTONE H3 (CENH3) occupancy and are densely DNA methylated, with satellite variants private to each chromosome. CENH3 preferentially occupies satellites that show the least amount of divergence and occur in higher-order repeats. The centromeres are invaded by ATHILA retrotransposons, which disrupt genetic and epigenetic organization. Centromeric crossover recombination is suppressed, yet low levels of meiotic DNA double-strand breaks occur that are regulated by DNA methylation. We propose that Arabidopsis centromeres are evolving through cycles of satellite homogenization and retrotransposon-driven diversification.
Související projekty:	Odhalení evolučních tajů lničky seté a příbuzných druhů