Informace o publikaci

OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice

Autoři	BAUMGARTNER D. JOHANNSEN B. SPECHT M. LUEDDECKE J. ROMBACH M. HIN S. PAUST N. VON STETTEN F. ZENGERLE R. HERZ Ch. PEHAM J.R. PAQUE P.N. ATTIN T. JENZER J.S. KOERNER P. SCHMIDLIN P.R. THURNHEER T. WEGEHAUPT F.J. KAMAN W.E. STUBBS A. HAYS J.P. RUSU V. MICHIE A. BINSL T. STEJSKAL D. KARPÍŠEK Michal BAO K. BOSTANCI N. BELIBASAKIS G.N. MITSAKAKIS K.
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	BIOSENSORS-BASEL
Fakulta / Pracoviště MU	Farmaceutická fakulta
Citace
www	https://www.mdpi.com/2079-6374/11/11/423
Doi	http://dx.doi.org/10.3390/bios11110423
Klíčová slova	dental practice; point-of-care diagnostics; treatment monitoring; oral health; periodontitis; caries; saliva diagnostics
Popis	first_page settings Open AccessArticle OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice by Desirée Baumgartner 1,2,, Benita Johannsen 1, Mara Specht 1, Jan Lüddecke 1, Markus Rombach 1, Sebastian Hin 1, Nils Paust 1,2, Felix von Stetten 1,2 [ORCID] , Roland Zengerle 1,2, Christopher Herz 3, Johannes R. Peham 3 [ORCID] , Pune N. Paqué 4 [ORCID] , Thomas Attin 4, Joël S. Jenzer 4 [ORCID] , Philipp Körner 4, Patrick R. Schmidlin 4 [ORCID] , Thomas Thurnheer 4 [ORCID] , Florian J. Wegehaupt 4 [ORCID] , Wendy E. Kaman 5,6, Andrew Stubbs 7 [ORCID] , add Show full author list 1 Hahn-Schickard, Georges-Koehler-Allee 103, 79110 Freiburg, Germany 2 Laboratory for MEMS Applications, IMTEK–Department of Microsystems Engineering, University of Freiburg, Georges-Koehler-Allee 103, 79110 Freiburg, Germany 3 AIT Austrian Institute of Technology, Molecular Diagnostics, Giefinggasse 4, 1210 Wien, Austria 4 Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Plattenstrasse 11, 8032 Zurich, Switzerland 5 Department of Medical Microbiology and Infectious Diseases, Erasmus University Medical Centre Rotterdam (Erasmus MC), 3015 CN Rotterdam, The Netherlands 6 Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Free University of Amsterdam and University of Amsterdam, 1081 LA Amsterdam, The Netherlands 7 Department of Pathology and Clinical Bioinformatics, Erasmus University Medical Centre Rotterdam (Erasmus MC), 3015 CN Rotterdam, The Netherlands 8 Magtivio B.V., Daelderweg 9, 6361 HK Nuth, The Netherlands 9 ClinicaGeno Ltd., 11 Station Approach, Coulsdon CR5 2NR, UK 10 Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, Syllabova 19, 70300 Ostrava, Czech Republic add Show full affiliation list Authors to whom correspondence should be addressed. Biosensors 2021, 11(11), 423; https://doi.org/10.3390/bios11110423 Received: 1 October 2021 / Revised: 22 October 2021 / Accepted: 24 October 2021 / Published: 28 October 2021 (This article belongs to the Special Issue Microfluidics for Biosensing) Download PDF Browse Figures Citation Export Abstract Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients’ general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk’s potential and suitability for inclusion in larger prospective implementation studies in dental care settings.