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Offline preparative separation methods based on electromigration: An overview and current trends

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HRUŠKOVÁ Helena ŘEMÍNEK Roman FORET František

Rok publikování 2024
Druh Článek v odborném periodiku
Časopis / Zdroj TrAC Trends in Analytical Chemistry
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.sciencedirect.com/science/article/pii/S0165993623005150
Doi http://dx.doi.org/10.1016/j.trac.2023.117428
Klíčová slova Electromigration; Preparative; Sample preparation; Electrophoresis; Free flow (free-flow) electrophoresis; Isoelectric focusing; Isotachophoresis; Matrix
Popis Sample preparation is undoubtedly one of the most essential steps in the analytical process. Generally, it facilitates or even enables subsequent measurement of the analytical signal. Electromigration techniques can be effectively used for the preparation of charged analytes. For these reasons, this review article summarizes the progress in offline electromigration preparative techniques between 2018 and 2023. The introduction includes a brief history of the development of electromigration preparative techniques and general theoretical insights into this area of knowledge. The subsequent text consists of four subchapters devoted one by one to electrophoresis, free-flow electrophoresis, isoelectric focusing, and isotachophoresis. Every part includes brief theoretical aspects of the specific electromigration technique and a discussion of the trends, developments, problems, and recently published studies providing a solution for diminishing these limitations.

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