Informace o publikaci
Crystallographic study of the maize cytokinin glucoside-specific beta-glucosidase Zm-p60.1
| Autoři | |
|---|---|
| Rok publikování | 2000 |
| Druh | Článek ve sborníku |
| Konference | 19-th European Crystallographic Meeting - Abstracts book |
| Fakulta / Pracoviště MU | |
| Citace | |
| Obor | Genetika a molekulární biologie |
| Popis | Zm-p60.1, a cytokinin-glucoside-specific b-glucosidase from maize, is a key enzyme involved in plant development and growth. It has been over-expressed in soluble form from Escherichia coli with a His tag at its N-terminus, the recombinant protein has been purified and crystallized at room temperature using PEG 4000 as main precipitant. A flash-annealed monoclinic crystal diffracted to high resolution (beyond 2 A) with space group P21, and unit-cell parameters a = 55.66 A, b = 110.72 A, c = 72.94 A, b = 92.10 deg. Resulting crystal structure was solved by molecular replacement. |
| Související projekty: |