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Analysis of chromosome termini in potato varieties

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FAJKUS Jiří NOVOTNÁ Marcela PTÁČEK Jiří

Rok publikování 2002
Druh Článek v odborném periodiku
Časopis / Zdroj ROSTLINNÁ VÝROBA
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Obor Genetika a molekulární biologie
Klíčová slova plant chromosomes; telomere length; potato varieties; terminal restriction fragments
Popis Chromosomes of potato are terminated by telomeres, which are formed by tandemly repeated [TTTAGGG]n oligonucleotide sequence. The total length of blocks of telomeric DNA has been known to vary largerly among plant species and their varieties, and also among individual chromosome arms within a single nucleus. To check for such differences in potato varieties, which could be of a possible use in genotyping, we performed pulsed-field gel electrophoretic analysis of terminal restriction fragments in selected potato varieties. We found a typical range of telomere lengths of 20-60 kb in most analysed varieties. In one of analysed varieties (Monalisa), telomeres of wider span (up to 80 kb) have been observed. Most of restriction enzymes (PvuII, HaeIII, TaqI) produced a resulting smeared hybridisation pattern of telomeres. When using BglII, however, a doublet hybridisation band could be observed. This may reflect differences in composition of telomere-associated sequences at different chromosome ends.
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