Soluble BAFF Levels Inversely Correlate with Peripheral B Cell Numbers and the Expression of BAFF Receptors

• Autoři: KREUZALER Matthias; RAUCH Melanie; SALZER Ulrich; BIRMELIN Jennifer; RIZZI Marta; GRIMBACHER Bodo; PLEBANI Alessandro; LOUGARIS Vassilios; QUINTI Isabella; THON Vojtěch; LITZMAN Jiří; SCHLESIER Michael; WARNATZ Klaus; THIEL Jens; ROLINK Antonius G.; EIBEL Hermann
• Rok publikování: 2012
• Druh: Článek v odborném periodiku
• Časopis / Zdroj: The Journal of Immunology
• Fakulta / Pracoviště MU: Lékařská fakulta
• www: http://www.jimmunol.org/cgi/doi/10.4049/jimmunol.1102321
• Doi: http://dx.doi.org/10.4049/jimmunol.1102321
• Obor: Epidemiologie, infekční nemoci a klinická imunologie
• Klíčová slova: chronic lymphocytic leukemia; Creactive protein; common variable immunodeficiency; primary antibody deficiency; systemic lupus erythematosus
• Popis: The TNF family member protein BAFF/BLyS is essential for B cell survival and plays an important role in regulating class switch recombination as well as in the selection of autoreactive B cells. In humans, increased concentrations of soluble BAFF are found in different pathological conditions, which may be as diverse as autoimmune diseases, B cell malignancies, and primary Ab deficiencies (PAD). Because the mechanisms that regulate BAFF levels are not well understood, we newly developed a set of mAbs against human BAFF to study the parameters that determine the concentrations of soluble BAFF in circulation. Patients with PAD, including severe functional B cell defects such as BTK, BAFF-R, or TACI deficiency, were found to have higher BAFF levels than asplenic individuals, patients after anti-CD20 B cell depletion, chronic lymphocytic leukemia patients, or healthy donors. In a comparable manner, mice constitutively expressing human BAFF were found to have higher concentrations of BAFF in the absence than in the presence of B cells. Therefore, our data strongly suggest that BAFF steady-state concentrations mainly depend on the number of B cells as well as on the expression of BAFF-binding receptors. Because most patients with PAD have high levels of circulating BAFF, the increase in BAFF concentrations cannot compensate defects in B cell development and function.

Související projekty

• Pathophysiology and Natural Course of Primary Antibody Deficiency (PAD) Syndromes