The same input data as for the QS WUR is applied to the assessment of universities in the various fields of science. In the current ranking published in April 2024, a total of 55 subjects and 5 broad subject areas were assessed: Arts & Humanities, Engineering & Technology, Life Sciences and Medicine, Natural Sciences and Social Sciences & Management. A total of 4,989 institutions from 149 countries were included in the assessment, and a total of 1,559 institutions were published in individual Subject Rankings.

Masaryk University ranked in a total of 13 subjects in the QS Subject Rankings published in 2024. Across the subjects, it performs best in the indicators Citations per Paper (especially for Environmental Sciences and Anatomy & Physiology) and H-Index (highest score for Politics & International Studies and then Law). It has strong Academic reputation in the Linguistics and Geography subject field and Employer Reputation Chemistry.

The highest overall score after weighting individual indicators was achieved by MU in Environmental Sciences (69.6), Geography (64.7 - only 230 institutions published), Education (63.1), and Archaeology (61.7).