Project information
Chronic myeloid leukemia radiation risk estimation based on BCR-ABL distances in hemotopoietic cells
- Project Identification
- GA202/02/0804
- Project Period
- 1/2002 - 12/2004
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Informatics
- Cooperating Organization
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Institute of Biophysics
- Responsible person Ing. Emilie Lukášová, CSc.
Our goal is to produce scientifically credible estimates of radiation-induced chronic myeloid leukemia (CML) risk. Ionizing radiation is a well-studied carcinogen known to cause CML and CML is well-undestood, homogenous and prevalent. CML is known to be caused by a translocation between ABL and BCR;almost all CML patients have the BCR-ABL translocation and CML induction is unequivocally detected in epidemiological studies. Using 3D confocal microscopy we shall measure BCR-to-ABL distances in human hematopoietic stem cells supposed to be the target cells of BCR-ABL translocation. BCR-to-ABL distance shorter than 0.5 micrometer was found in 3% to 20 % of human lymphocytes. Since this figure is much higher than expected for independent loci, BCR and ABL appear to be spatially correlated in some cells. This correlation can increase the frequency of BCR-ABL translocation if present also in target progenitor cells. Data obtained in these cells will represent zero-dose data and will be relevant to CML r
Publications
Total number of publications: 10
2006
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Directional motion of foreign plasmid DNA to nuclear HP1 foci
Chromosome Research, year: 2006, volume: 14, edition: 5
2004
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Automated image analysis in fluorescence microscopy: From isolated cells to tissues and microarray images
Biophysics of the Genome, year: 2004
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Colocalization of PML bodies and PML/RARalpha microspeckles with up- and down-regulated loci and changes of chromatin structure in APL leukemia cells
Biophysics of the Genome, year: 2004
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From Jenalumar to Ultrafast Microscopy of Living Cells
Biophysics of the Genome, year: 2004
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Gene expression in colorectal carcinoma determined by cDNA microarrays using a combination of several evaluation approaches.
Biophysics of the Genome, year: 2004
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Methylation of histone H3K9 in human blood cells and in granulocytes of patients with myeloid leukemia
Biophysics of the Genome, year: 2004
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Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Year: 2004, type: Conference abstract
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Topography of genetic loci in the nuclei of cells of colorectal carcinoma and adjacent tissue of colonic epithelium
Chromosoma, year: 2004, volume: 112, edition: 5
2003
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Nuclear and territorial topography of chromosome telomeres in human lymphocytes
Experimental Cell Research, year: 2003, volume: 289, edition: 1
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Topography of telomeres and structure of chromosome territories in human lymphocytes
Biophysics of the Genome, year: 2003