Project information
Nové flavin-dependentní enzymy Paracoccus denitrificans: reakční mechanismy, metabolické funkce a úloha v buněčném oxidačním stresu
- Project Identification
- GAP503/12/0369
- Project Period
- 1/2012 - 12/2015
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
Projekt se zabyva dvema enzymy,
avin reduktasou FerA a chinonreduktasou FerB, spojenymi s genero-
vanm nebo zvladanm oxidacnho stresu u bakterie Paracoccus denitri cans, casto nazyvane
"
volne zijc
mitochondrie\. Bude izolovan gen pro FerA a pripraveny odpovdajc rekombinantn protein bude charak-
terizovan enzymologicky, krystalogra cky a pomoc mstne rzene mutageneze. Bude stanoveno, zda FerA
pusob samostatne nebo v kombinaci s dalsmi proteiny. V pokusech in vitro bude kvanti kovana produkce
superoxidu enzymem FerB a testovana hypoteza, ze FerB je schopen redukovat membranovy ubichinon. U divokeho kmene a mutantnch kmenu bez FerA resp. FerB nebo s jejich zvysenou produkc bude porov-
nan stupen peroxidace membranovych lipidu. Proteomicka analyza bunek vystavenych redoxnm stresorum
pomuze pri identi kaci clenu redoxnho regulonu.
Publications
Total number of publications: 6
2017
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An Enzymatic Method for Methanol Quantification in Methanol/Ethanol Mixtures with a Microtiter Plate Fluorometer
Food Analytical Methods, year: 2017, volume: 10, edition: 5, DOI
2016
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Biochemical properties and crystal structure of the flavin reductase FerA from Paracoccus denitrificans
Microbiological Research, year: 2016, volume: 188, edition: 1, DOI
2015
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Proteome-wide dataset generated by iTRAQ-3DLCMS/MS technique for studying the role of FerB protein in oxidative stress in Paracoccus denitrificans
Data in Brief, year: 2015, volume: 4, edition: September, DOI
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Proteomic responses to a methyl viologen-induced oxidative stress in the wild type and FerB mutant strains of Paracoccus denitrificans
Journal of Proteomics, year: 2015, volume: 125, edition: July, DOI
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The flavoprotein FerB of Paracoccus denitrificans binds to membranes, reduces ubiquinone and superoxide, and acts as in vivo antioxidant
FEBS Journal, year: 2015, volume: 282, edition: 2, DOI
2014
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The Structural and Functional Basis of Catalysis Mediated by NAD(P)H:acceptor Oxidoreductase (FerB) of Paracoccus denitrificans
PLOS ONE, year: 2014, volume: 9, edition: 5, DOI