Project information
Topographic and functional characterization of telomeres in various tumor cell lines determined in situ and in vivo
- Project Identification
- GP204/05/P090
- Project Period
- 1/2005 - 12/2007
- Investor / Pogramme / Project type
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Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
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Faculty of Informatics
- Mgr. Ing. Jana Amrichová, Ph.D.
- Keywords
- topography of telomeres;telomere association;telomere dynamics;ALT;nuclear architecture;apoptosis;cell cycle;telomerase;tumor cell
All human tumors as well as immortalized cell lines, which have been analyzed so far, can be characterized by mechanisms maintaining viable length of telomeres and thus stabilizing malignant and immortalized phenotype of these cells. The ribonucleoprotein enzyme, known as telomerase, elongates telomeres in most tumors (85 to 90%) by the process of reverse-transcription. On the other hand, there are rare telomerase-negative tumors, which are provided with alternative mechanism for telomere elongation based on inter-telomeric homologous recombination. The presented project will concentrate on detailed in situ and in vivo analysis of topography and dynamics of telomeres during cell cycle, apoptosis, and after cell fusion in healthy cells and tumor cell lines with various mechanisms of telomere elongation. This structural analysis will be supplemented with functional analysis of telomeres; i.e. measurement of telomere length and level of telomerase expression and activity. Obtained results will help us better understand the origin and regulation of tumorigenesis and cellular immortalization and thus can lead to discovery of new therapies for cancer treatment.
Results
Publications
Total number of publications: 8
2009
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Prediction of localization and interactions of apoptotic proteins
Journal of Biomedical Science, year: 2009, volume: 16, edition: 59
2008
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Bioinformatic prediction, molecular modelling and image analysis of localization and interactions of apoptotic proteins endonuclease G, AIF, and AMID
Year: 2008, type: Conference abstract
2007
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Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells
Apoptosis, year: 2007, volume: 12, edition: 7
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Bioinformatic predictions and image analysis of localization and interactions of endonuclease G, AIF, and AMID in human cells
Year: 2007, type: Conference abstract
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Spatial Organization, Dynamics, and Associations of Telomeres in Healthy, Telomerase-Positive and ALT Human Cell Lines
Year: 2007, type: Conference abstract
2006
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Analýza lokalizace a interakce fluorescenčně značených molekul v živých i fixovaných lidských buňkách.
Year: 2006, type: Conference abstract
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Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
Year: 2006, type: Conference abstract
2005
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Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
Year: 2005, type: Conference abstract