Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
-
Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
-
Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 834
2011
-
Fast methods of atomic charge calculation: the Electronegativity Equalization Method for proteins.
Year: 2011, type: Conference abstract
-
Fe/Mn superoxide dismutase in Paracoccus denitrificans is induced by azide and expressed independently on FNR-type regulators
Folia Microbiologica, year: 2011, volume: 56, edition: 1, DOI
-
Function and structure studies of lectin from bioluminescent bacterium Photorhabdus luminescens
Year: 2011, type: Conference abstract
-
Generic Method for Cytochrome P450 Metabolism Studies Employing Short End Injection Capillary Electrophoresis Mode
Year: 2011, type: Conference abstract
-
High-fat diet-induced changes in thyroid hormones metabolism in murine white adipose tissue: Ascertainment by radiometric enzyme assays
Year: 2011, type: Conference abstract
-
Host cell recognition by carbohydrate-binding proteins from human pathogens
Year: 2011, type: Conference abstract
-
How to calculate binding energy in Protein-Carbohydrate complexes
Year: 2011, type: Conference abstract
-
Changes in thyroid hormones metabolism in mice associated with high-fat diet-induced obesity
Year: 2011, type: Conference abstract
-
Chemical Shift Tensors in Isomers of Adenine: Relation to Aromaticity of Purine Rings?
ChemPhysChem, year: 2011, volume: 12, edition: 2, DOI
-
In vitro protein and metabolic engineering of a biodegradation pathway
Year: 2011, type: Appeared in Conference without Proceedings