Project information
A role of certain transcription factors in the osteoclastic differentiation pathway
- Project Identification
- GA301/06/0036
- Project Period
- 1/2006 - 12/2008
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
-
Faculty of Science
- prof. RNDr. Jan Šmarda, CSc.
- Mgr. Petr Beneš
- prof. Pierre Jurdic
- Mgr. Jarmila Navrátilová, Ph.D.
- Mgr. Vendula Pelková
- doc. RNDr. Petr Vaňhara, Ph.D.
- Keywords
- Myb, Fos, Pu-1, MITF, osteoclast, differentiation, monocyte, macrophage, hematopoiesis
Osteoporosis is one of the leading causes of morbidity in the elderly and is characterized by a progressive loss of total bone mass and bone density. Bone loss in osteoporosis is due to the persistent excess of osteoclastic bone resorption over osteoblastic bone formation. Osteoclasts are cells of hematopoietic origin and their formation is controlled by multiple transcription factors. The aim of this proposal is to define the role of PU.1, MITF, Myb, Fos and NFB transcription factors in regulation of monoblasts/macrophages towards osteoclasts. The project is based (1) on analyses of expression of these transcription factors during induced differentiation of the cells of monoblastic (BM2) and macrophage (RAW) cell lines, (2) on targeted induction or repression of expression of the genes coding for these transcription factors in BM2 and RAW cells and detailed analyses of the effects resulting from changed expression profiles on differentiation capabilities of these cells. The conlusions about the function of the transription factors in control of osteoclast differentiation made on established cell lines are going to be verified on primary FL cells, that can be induced to differentiate towards dendritic cells, macrophages, microglial cells and osteoclasts by various growth factors. The elucidation of molecular mechanisms governing bone-cell differentiation is of particular importance considering the incidence and severity of the diseases that affect skeletal development.
Publications
Total number of publications: 28
2008
-
Heavy metals induce phosphorylation of the Bcl-2 protein by Jun N-terminal kinase
XIX. Biologické dny. Biologický výzkum pro lidské zdraví, year: 2008
-
Proteiny rodiny TGFbeta sekretované prostatickými nádorovými buňkami ovlivňují diferenciaci osteoklastů
Zborník abstraktov, year: 2008
-
Regulation of osteoclast differentiation by the TGFbeta proteins secreted by prostate cancer
Stem cells, cancer and aging, year: 2008
-
The effects of TGFb proteins on osteoclast differentiation
Proceedings of the abstracts of the 13th World Congress on Advances in Oncology and 11th International Symposium on Molecular Medicine, year: 2008
2007
-
A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts
Leukemia Research, year: 2007, volume: 31, edition: 2
-
c-Jun induces apoptosis of starved BM2 monoblasts by activating cyclin A-CDK2
Biochemical and Biophysical Research Communications, year: 2007, volume: 353, edition: 1
-
Interakce okadaické a retinové kyseliny při kontrole diferenciace monoblastů transformovaných onkogenem v-myb
XI. Setkání biochemiků a molekulárních biologů, year: 2007
-
Lidský nádorový supresor p53 zastavuje růst a indukuje diferenciaci ptačích monoblastů
Edukační sborník, year: 2007
-
p53 arrests growth and induces differentiation of v-Myb-transformed monoblasts
Differentiation, year: 2007, volume: 75, edition: 7
-
p53 arrests growth and induces differentiation of v-myb-transformed monoblasts
Molecular Aspects of myeloid stem cell development and leukemia, year: 2007