Project information
Mechanism of procathepsin D effect on breast cancer cells
- Project Identification
- IAA501630801
- Project Period
- 1/2008 - 12/2011
- Investor / Pogramme / Project type
-
Academy of Sciences of the Czech Republic
- Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the ASCR
- MU Faculty or unit
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Faculty of Science
- doc. Mgr. Petr Beneš, Ph.D.
- Mgr. Lucia Knopfová, Ph.D.
- Mgr. Jarmila Navrátilová, Ph.D.
- Mgr. Eva Ondroušková, Ph.D.
- prof. RNDr. Jan Šmarda, CSc.
- Keywords
- procathepsin D, breast cancer, protein interactions, apoptosis, cell signaling
Procathepsin D (pCD) is over-expressed and secreted by several types of tumors and cancer cell lines. Increased level of pCD is known to affect multiple stages of tumor progression, including proliferation, invasion and metastasis and is associated with poor prognosis of various cancers. It has been suggested that protein-protein interactions are important mediators of these effects. Using gene microarray approach we have recently identified several differentially expressed genes upon treatment of breast cancer cells with pCD. This project is therefore designed to A) explore the role of NF-kB2 and MAPK in mediating the effects of pCD on breast cancer cells, B) search for pCD protein-protein interacting partners, C) analyze the role of pCD-mediated signaling in regulation of apoptosis of breast cancer cells. We believe that this project can answer the question how the pCD affects cancer cells. Full understanding of this mechanism is important for design of pCD-targeted cancer therapy.
Publications
Total number of publications: 20
2012
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Redox State Alters Anti-Cancer Effects of Wedelolactone
Environmental and Molecular Mutagenesis, year: 2012, volume: 53, edition: 7, DOI
2011
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Cathepsin D expression as a regulator of breast cancer cell migration and chemosensitivity
Year: 2011, type: Conference abstract
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c-Myb regulates MMP1, MMP9 and cathepsin D: Implications for the matrix-dependent breast cancer cell invasion and metastasis
Year: 2011, type: Conference abstract
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c-Myb-induced migration/invasiveness of breast and colon cells in vitro is not associated with enhanced lung colonization in vivo
Year: 2011, type: Conference abstract
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Inhibition of topoisomerase IIa: Novel function of wedelolactone
Cancer Letters, year: 2011, volume: 303, edition: 1, DOI
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OVEREXPRESSION OF CATHEPSIN D SENZITIZES BREAST CANCER CELLS TO TRAIL-INDUCED APOPTOSIS
Year: 2011, type: Conference abstract
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The effects of wedelolactone on cancer cells depend on its redox state
Year: 2011, type: Conference abstract
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The Overexpression of Cathepsin D Senzitizes Brest Cancer Cells to TRAIL-induced Apoptosis
Year: 2011, type: Conference abstract
2010
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c-Myb promotes invasivity of breast cancer cells
Year: 2010, type: Conference abstract
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c-Myb promotes motility and invasion of breast cancer cells
Year: 2010, type: Conference abstract