Project information
In vitro methods for detection and studies of epigenetic toxicity of xenobiotics
- Project Identification
- GA525/00/D101
- Project Period
- 1/2000 - 1/2003
- Investor / Pogramme / Project type
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Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
Polycyclic aromatic hydrocarbons (PAHs), pesticides and other compounds belong among the most important environmental pollutants. Although many mechanisms of their toxic effects were recognized (mutagenicity, immunotoxicity), new evidences show that mech anisms of epigenetic toxicity affecting processes of cancerogenesis can be involved. Project propose development, evaluation and application of the battery of in vitro tests for detection of epigenetic mechanisms of cellular and molecular toxicity. Some of these tests are still omitted of completely missing. Following are the in vitro tests included in the proposed battery: multiparametric system for studies of oxidative stress (ROS production, GSH depletion, oxidative DNA damage, lipid peroxidation), a pplication of the methods of receptor-mediated toxicity (AhR, ER) and development of new methods (PPAR), research of inhibitors of intercellular gap juntional communications (GJIC) and development of models for studies of detoxifying enzymes (CYPIA, CYP1
Publications
Total number of publications: 23
2004
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Geosmin occurrence in riverine cyanobacterial mats: is it causing a significant health hazard?
Water Science and Technology, year: 2004, volume: 49, edition: 1
2003
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In vitro inhibition of GJIC in WB-F344 cells by polar derivatives of PAHs.
SETAC Europe 13th Annual Meeting, year: 2003
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Mobility, bioavailability and toxic effects of cadmium in soil samples.
Environmental Research, year: 2003, volume: 91, edition: -
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Novel retinoid-specific luciferase reporter gene bioassay for screening of embryotoxic potential. Persistent Toxic Substances Contamination of the European Region
RECETOX EU-Center of Excellence 1st Workshop, year: 2003
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PCB in aquatic ecosystems of the River Elbe and Berlin waters - source oriented monitoring.
Fresenius Environmental Bulletin, year: 2003, volume: 12, edition: -
2002
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Acute inhibition of gap junctional intercellular communication by polychlorinated biphenyls and their metabolites - relative inhibitory potencies, structure-activity relationship and intracellular singnalling
Book of Abstracts - International Summer School, NATO/FEBS Advanced Study Institute - Spetses, Greece, 18-30/8/2002, year: 2002
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Comparison of AhR and ER mediated activities of hydroxyderivatives of polychlorinated biphenyls detected in vitro with luciferase reporter gene techniques
Book of Abstracts - The 2nd PCB Workshop: Recent Advances in the Environmental Toxicology and Health Effects of PCBs Emphasis on the latest advances and the perspective of Central and Eastern Europe. Brno, CR, 07-11/05/2002, year: 2002
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Comparison of AhR and ER mediated activities of hydroxyderivatives of polychlorinated biphenyls detected in vitro with luciferase reporter gene techniques
The Second PCB Workshop. Recent Advances in the Environmental Toxicology and Health Effects of PCBs. Book of Abstracts, year: 2002
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Comparison of relative toxic potencies of hydroxylated PCBs and quinones in hepatic and mammary cell lines
Organohalogen Compounds, year: 2002, volume: 56, edition: 1
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Effects of cyanobacterial biomass and purified microcystins on malformations in Xenopus laevis: teratogenesis assay (FETAX)
Environmental Toxicology, year: 2002, volume: 6, edition: 17