Project information
Building Barbara McClintock’s legacy: Evolution of ribosomal DNA in chromosomal context

Ribosomal RNA genes (rDNAs) have long fascinated researchers, representing a captivating enigma within molecular biology. Despite relatively small genomic fraction, rDNAs wield disproportionate influence, orchestrating crucial cellular processes as the architects of ribosomes. Delving into the mechanisms governing DNA mobility already defined by Barbara McClintock almost a century ago offers a gateway to transformative discoveries with far-reaching implications across various fields. Our enduring fascination with rDNAs dynamism in chromosomes has motivated us to re-open old rDNA-related questions using state-of-the-art techniques, such as T2T chromosome assemblies, 3D chromosome painting and genome editing. Specifically, we will investigate the relationship between the position of rDNA in chromosomes, their mobility, copy number, homogenization (concerted evolution), epigenetic modification and expression. The generated results will likely contribute to our better understanding of the genome function and evolution.