Project information
Peripheral blood-derived human monocytes/macrophages as a model to study genotype-specific differences in RAGE signalling

This project will be funded by The Guildford Bench Methodology Fund. The purpose of the grant/project is: 1) To establish isolation of human monocytes from the peripheral venous blood and reproducible yield from cubital venepuncture of max. 15ml of blood. 2) To accommodate an existing differentiation protocol into macrophages for our settings under both of normo- and hyperglycemia conditions (5 and 15mM/L glucose culture medium) and test for viability of such primary cultures. 3) To verify expression of surface RAGE and production of soluble RAGE into culture media (using specific anti-human monoclonal antibodies).