Project information
Study of the molecular mechanisms of biodegradation reactions - construction of QSBR models and protein engineering of haloalkane dehalogenases
- Project Identification
- GA203/97/P149
- Project Period
- 1/1998 - 1/2000
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
- Faculty of Science
The project is focused on biodegradation of the organic compounds by microorganisms. Fundamental mechanisms of the biodegradation reaction and the molecular level will be studied. The methods of Quantitative Structure-Biodegradability Relationships (QSBR ) a in vitro modifications of the proteins will be employed. These methods will be used to investigate the biological degradation of halogenated aliphatic compounds which belong to common groundwater and soil contaminants. Mathematical models serving for the predictions of biodegradability of halogenated compounds will be constructed within the project. These models can find their use in the Environmental Risk Assessment. Modification in the enzymes haloalkane dehalogenases will be proposed using the co mputer modelling. These modifications should lead towards better activity and/or broader specificity of the dehalogenating enzymes.
Publications
Total number of publications: 15
2001
-
Reaction mechanism and stereochemistry of gamma-hexachlorocyclohexane dehydrochlorinase LinA
Journal of Biological Chemistry, year: 2001, volume: 276, edition: 11
-
TRITON: graphic software for rational engineering of enzymes
Trends in Biochemical Sciences, year: 2001, volume: 28, edition: 1
2000
-
Dehalogenation of haloalkanes by Mycobacterium tuberculosis H37Rv and other mycobacteria
Applied and Environmental Microbiology, year: 2000, volume: 66, edition: 1
-
Determination of haloalkane dehalogenase enzymatic activity by capillary zone electrophoresis
Book of Abstract "13th International symposium on high performance capillary electrophoresis and related techniques HPCE 2000"., year: 2000
-
Determination of haloalkane dehalogenase enzymatic activity by capillary zone electrophoresis
Journal of Chromatography A, year: 2000, volume: 895, edition: 1-2
-
Effect of the carbon source on assessment of degrading bacteria with the spread-plating technique during in situ bioremediation
Folia Microbiologica, year: 2000, volume: 45, edition: 1
-
Rapid detection of haloalkane dehalogenase activity by capillary zone electrophoresis
Chemické Listy, year: 2000, volume: 94, edition: 8
-
TRITON: in silico construction of protein mutants and prediction of their activities
Bioinformatics, year: 2000, volume: 16, edition: 9
1999
-
Analysis of the reaction mechanism and substrate specificity of haloalkane dehalogenases by sequential and structural comparisons
Protein Engineering, year: 1999, volume: 12, edition: 11
-
Construction and characterization of histidine-tagged haloalkane dehalogenase (LinB) of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, Sphingomonas paucimobilis UT26
Protein Expression and Purification, year: 1999, volume: 17, edition: 2