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Publication details
Vztah rodičovských genomů u mezidruhových hybridů Trifolium pratense x Trifolium medium
Title in English | Intergenomic relationships in interspecific hybrids of Trifulium pratense x Trifolium medium |
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Authors | |
Year of publication | 2012 |
Type | Article in Periodical |
Magazine / Source | Úroda, Vědecká příloha |
MU Faculty or unit | |
Citation | |
Field | Genetics and molecular biology |
Keywords | Flow cytometry Genomic in situ hybridisation Trifolium medium Trifolium pratense |
Description | Breeding material derived from hybrids Trifolium pratense x T. medium is a promising source of new genetic variability for cultivated red clover (7. pratense). These hybrids were evaluated on the cytological level via flow cytometry and genomic in situ hybridization. Plants of 99 sources in two breeding nurseries had comparable chromosome numbers in five groups: 2n : 28 (76 and 66% of plants), bellow of 28 chromosomes (8.5 and6.4oh of plants),2n:29,30 (15 and 26% of plants),2n: 31, 40 (0.4 and 1.2% of plants) and 2n: 4I to 46 (0.1 and 0.3% of plants). The identification of introduced chromosomes or translocated parts was unsuccessful. Hybrids contained lover level of phytoestrogens than T. pratense but increased amount of formononetin with comparison of T. medium was evaluated. Interspecific variability will be identified by genomic procedures based on de novo sequencing of the both parental genome's. |
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