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Publication details
Genetický marker RGH1aI1a pro rezistenci k původci padlí travního u ječmene
Title in English | Genetic marker RGH1aI1a for powdery mildew resistance in barley |
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Authors | |
Year of publication | 2012 |
MU Faculty or unit | |
web | http://www.akademiai.com/content/r501324342214455/fulltext.pdf |
Description | DNA marker is developed from the sequence of the RGH1a gene (intron 1) at the Mla locus on chromozomu 1H in barley (Hordeum vulgare). The primer sequences (5`-GCCTCATCTGACCGTTGATT-3`, 5`-GCATCACTGCCATCTCTATGC-`3) and amplification cycle were designed for detection of target sequence. Amplified DNA fragment 490 bp is detected by gel electrophoresis. Functional sample was made by verifying of properties. |
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