Publication details
Genetický marker RGH1aE2I2 pro rezistenci k původci padlí travního u ječmene
| Title in English | Genetic marker RGH1aE2I2 for powdery mildew resistance in barley |
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| Authors | |
| Year of publication | 2012 |
| MU Faculty or unit | |
| web | http://download.springer.com/static/pdf/680/art%253A10.1007%252Fs10681-009-0096-0.pdf?auth66=1362648435_e00056c0c6e061a50e4e4e7bdfa5604a&ext=.pdf |
| Description | DNA marker is developed from the sequence of the RGH1a gene (exon 2 and intron 2) at the Mla locus on chromozomu 1H in barley (Hordeum vulgare). The primer sequences (5`-CTGCTAGTCATCCGTGCAGA-3`, 5`-ACGATTTGTGTGAGCACGAG-`3) and amplification cycle were designed for detection of target sequence. Amplified DNA fragment 506 bp is detected by gel electrophoresis. Functional sample was made by verifying of properties. |
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