Publication details

Enterococcus ureilyticus sp. nov. and Enterococcus rotai sp. nov., two urease-producing enterococci from the environment

Authors

SEDLÁČEK Ivo HOLOCHOVÁ Pavla MAŠLAŇOVÁ Ivana KOSINA Marcel SPRÖER Cathrin BRYNDOVÁ Hana VANDAMME Peter RUDOLF Ivo HUBÁLEK Zdeněk ŠVEC Pavel

Year of publication 2013
Type Article in Periodical
Magazine / Source International Journal of systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1099/ijs.0.041152-0
Field Microbiology, virology
Keywords Enterococcus; taxonomy; description
Description A set of 25 urease producing, yellow pigmented enterococci was isolated from environmental sources and studied by using a polyphasic taxonomic approach. The phenotypic classification divided the isolates into two groups and both phena were characterized using 16S rRNA gene sequence analysis, DNA base composition, rep-PCR fingerprinting and automated ribotyping. The obtained data distinguished the isolates from all validly named Enterococcus spp. and were placed in the Enterococcus faecalis species group. DNA-DNA hybridisation experiments, pheS and rpoA genes sequencing, and whole-cell protein electrophoresis provided conclusive evidence for the classification of both phena as novel Enterococcus species for which the names Enterococcus ureilyticus sp. nov. (type strain CCM 4629T = LMG 26676T = CCUG 48799T), inhabiting water and plants, and Enterococcus rotai sp. nov. (type strain CCM 4630T = LMG 26678T = CCUG 61593T), inhabiting water, insects (mosquitoes) and plants are proposed
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