Publication details
Immobilized Enzyme Reactor for On-Line Studies of Drug Metabolism Mediated by Cytochrome P450 2C9 Isoform
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| web | http://www.chemicke-listy.cz/docs/full/2013_s3_s420-s453.pdf |
| Field | Biochemistry |
| Keywords | Capillary Electrophoresis; Drug Metabolism; CYP2C9; Diclofenac; Immobilization; IMER |
| Description | Drug biotransformation mediated by cytochrome P450 enzymes (CYP) is a pivotal factor in the early developmental stages of new drugs. Metabolism appraisal of every hit thus constitutes the inseparable part of ADME/tox screenings. Enzyme immobilization represents a useful approach in this field because of CYP recycling and simplification of sequential analyses by providing protein-free samples. A new method for immobilization of therapeutically important cytochrome P450 2C9 isoform (CYP2C9) on magnetic microparticles SiMAG Carboxyl is thus presented. |
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