X ray vs. NMR structure of the N terminal domain of delta subunit of RNA polymerase

• Authors: DEMO Gabriel; PAPOUŠKOVÁ Veronika; KOMÁREK Jan; KADEŘÁVEK Pavel; OTRUSINOVÁ Olga; SRB Pavel; RABATINOVA Alzbeta; KRASNY Libor; ŽÍDEK Lukáš; SKLENÁŘ Vladimír; WIMMEROVÁ Michaela
• Year of publication: 2013
• Type: Conference abstract
• MU Faculty or unit: Central European Institute of Technology
• Description: The crystal structure of the N terminal domain of delta subunit of RNA polymerase from Bacillus subtilis solved at the resolution of 2.0 A is presented. The X ray structure shows significant differences from the average NMR structure. We systematically investigated the cause of the discrepancies between the NMR and X ray structures, as the pH, presence of metal ions and crystal packing forces.

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• CEITEC - Central European Institute of Technology