Permanent Genetic Resources added to Molecular Ecology Resources Database 1 April 2013-31 May 2013

• Authors: AGOSTINI Cecilia; ALBALADEJO R.G.; APARICIO A.; ARTHOFER Wolfgang; BERREBI P.; BOAG Peter T.; CARBONE Ignazio; CONROY Gabriel C.; CORTESERO A.M.; GONCALVES Evonnildo Costa; COSTA Diogo; COUTO Alvarina; DE GIROLAMO Mirko; DU Hao; FU Shi-Jian; GARRIDO-GARDUNO T.; GETTOVÁ Lenka; GILLES A.; HAMOY Igor Guerreiro; HERRERA C. M; HEUSSLER Carina; ISIDRO Eduardo; JOSSO C.; KRAPF Patrick; LAMONT Robert W.; LE RALEC A.; LOPES Susana; LUIS Carla; LUO Hui; MAHEO F.; MARINO Ilaria A. M.; MIEUZET L.; MURRAY Brent W.; OGBOURNE Steven M.; PALLAVICINI Alberto; PAREJO-FARNES C.; PATARNELLO Tomaso; PATY C.; PEREIRA Carolina; PINHO Catarina; PINTO Pablo; POINSOT D.; POWELL Adrienne; PUTMAN Alexander I.; SANTORO Andre; SANTOS Sidney; SCHLICK-STEINER Birgit C.; SCOTT Candace; BARBOSA Maria Silvanira; VETEŠNÍKOVÁ ŠIMKOVÁ Andrea; SIMON J. -C.; SOLE-CAVA Antonio; STEINER Florian M.; SUN Zhengxin; TORBOLI Valentina; TREDWAY Lane P.; DE GROOT Peter J. Van Coeverden; VASCONCELLOS Anderson; VAZQUEZ-DOMINGUEZ E.; WANG Deng-Qiang; WANG Yu-Xiang; WEI Qi-Wei; ZANE Lorenzo; ZHANG Shu-Huan
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: Molecular Ecology Resources
• MU Faculty or unit: Faculty of Science
• Doi: http://dx.doi.org/10.1111/1755-0998.12140
• Field: Genetics and molecular biology
• Keywords: microsatellites 454 GS-FLX Titanium sequencing cyprinids Barbus cross-amplification hybrid zones
• Description: Using cost-effective 454 GS-FLX Titanium sequencing technology, microsatellite markers were isolated de novo for Barbus meridionalis. Five multiplex sets including 28 newly and two previously-developed loci were optimized using allopatric populations of both B. barbus and B. meridionalis. Our study revealed the high success rate of cross-species amplification in two Balkan species, B. cyclolepis and B. balcanicus.

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