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Publication details
Metody izolace vybraných proteinů hlíz brambor
Title in English | Methods for isolating proteins of potato tubers |
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Authors | |
Year of publication | 2013 |
MU Faculty or unit | |
Description | The basic element of the separation process is a two step procedure based on ion exchange and affinity chromatography. For subsequent fractionation a column with ceramic media and gradient elution via FPLC is used. The aim of the methodology is the determination of two fundamental biological activities of proteins tubers - lipidacylhydrolase patatin activity and activities of protease inhibitors. |
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