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Minimum aeration in Acidithiobacillus ferrooxidans cultures required to maintain substrate oxidation without oxygen limitation
Authors | |
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Year of publication | 2013 |
Type | Article in Proceedings |
Conference | 20th International Biohydrometallurgy Symposium, IBS 2013 |
MU Faculty or unit | |
Citation | |
Doi | http://dx.doi.org/10.4028/www.scientific.net/AMR.825.414 |
Field | Mining industry incl. coal mining and processing |
Keywords | Acidithiobacillus ferrooxidans; aeration; iron-oxidising bacteria; oxygen limitation; sulphur-oxidizing bacteria; volumetric oxygen transfer coefficient |
Description | The volumetric oxygen transfer coefficient (kLa) was used to define the conditions necessary for minimum aeration and to eliminate potential oxygen limitation in bioleaching cultures of Acidithiobacillus ferrooxidans. The Michaelis constants for oxygen were 1.07 and 0.71 umol O2/l for the oxidation of ferrous iron and elemental sulphur, respectively. The critical oxygen concentration, below which oxygen limitation occurred, was determined to be 6.250 and 3.125 umol O2/l for the oxidation of ferrous iron and elemental sulphur, respectively. The (kLa)crit values required to maintain oxygen-unlimited substrate oxidation for ferrous iron and elemental sulphur were 7.70 and 4.88/h, respectively. |