Benzofurazane as a New Redox Label for Electrochemical Detection of DNA: Towards Multipotential Redox Coding of DNA Bases

• Authors: BALINTOVÁ Jana; PLUCNARA Medard; VIDLÁKOVÁ Pavlína; POHL Radek; HAVRAN Luděk; FOJTA Miroslav; HOCEK Michal
• Year of publication: 2013
• Type: Article in Periodical
• Magazine / Source: Chemistry - A European Journal
• MU Faculty or unit: Central European Institute of Technology
• web: http://onlinelibrary.wiley.com/doi/10.1002/chem.201301868/abstract;jsessionid=EF409FC4C3D959BEA050AC3B14DB1BA6.f01t04
• Doi: http://dx.doi.org/10.1002/chem.201301868
• Field: Biochemistry
• Keywords: DNA polymerase; electrochemistry; nucleoside triphosphates; sequencing; voltammetry
• Description: Benzofurazane has been attached to nucleosides and dNTPs, either directly or through an acetylene linker, as a new redox label for electrochemical analysis of nucleotide sequences. Primer extension incorporation of the benzofurazane-modified dNTPs by polymerases has been developed for the construction of labeled oligonucleotide probes. The combination of benzofurazane and nitrophenyl reducible labels has proved to be excellent for ratiometric analysis of nucleotide sequences and is suitable for bioanalytical applications.

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