Publication details
Vinylsulfonamide and Acrylamide Modification of DNA for Cross-linking with Proteins
| Authors | |
|---|---|
| Year of publication | 2013 |
| Type | Article in Periodical |
| Magazine / Source | Angewandte Chemie International Edition |
| MU Faculty or unit | |
| Citation | |
| Web | http://onlinelibrary.wiley.com/doi/10.1002/anie.201303577/abstract |
| Doi | http://dx.doi.org/10.1002/anie.201303577 |
| Field | Biochemistry |
| Keywords | bioorthogonal chemistry; DNA; DNA polymerase; Michael additions; proteins |
| Description | Bioorthogonal covalent cross-linking of DNA-binding proteins (p53) to DNA was achieved through novel DNA probes bearing a reactive vinylsulfonamide (VS) group. The VS-modified dCTP served as building block for polymerase synthesis of modified DNA, which was readily conjugated with cysteine-containing peptides and proteins by Michael addition. |