Publication details

Ribozyme Activity of RNA Nonenzymatically Polymerized from 3 ',5 '-Cyclic GMP

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Authors

PINO Samanta COSTANZO Giovanna GIORGI Alessandra ŠPONER Jiří ŠPONEROVÁ Judit DI MAURO Ernesto

Year of publication 2013
Type Article in Periodical
Magazine / Source Entropy
MU Faculty or unit

Central European Institute of Technology

Citation
web http://www.mdpi.com/1099-4300/15/12/5362
Doi http://dx.doi.org/10.3390/e15125362
Field Biophysics
Keywords RNA; RNA polymerization; RNA origin; ribozymes; origin of life
Description 3',5'-Cyclic GMP spontaneously nonenzymatically polymerizes in a base-catalyzed reaction affording G oligonucleotides. When reacted with fully or partially sequence-complementary RNA (oligo C), the abiotically generated oligo G RNA displays a typical ribozyme activity consisting of terminal ligation accompanied by cleavage of an internal phosphate site of the donor oligonucleotide stem upon attack of the acceptor 3' terminal OH. This reaction is dubbed Ligation following Intermolecular Cleavage (LIC). In a prebiotic perspective, the ability of oligo G polynucleotides to react with other sequences outlines a simple and possible evolutionary scenario based on the autocatalytic properties of RNA.
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