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Publication details
Staphylococcus aureus in Czech cystic fibrosis patients – prospective study
Authors | |
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Year of publication | 2014 |
Type | Article in Periodical |
Magazine / Source | Journal of Cystic Fibrosis |
MU Faculty or unit | |
Citation | |
web | http://www.sciencedirect.com/science/article/pii/S1569199314602320 |
Doi | http://dx.doi.org/10.1016/S1569-1993(14)60232-0 |
Field | Genetics and molecular biology |
Keywords | Staphylococcus aureus; small colony variants; cystic fibrosis; enterotoxin genes |
Description | Altogether, 242 respiratory isolates of S. aureus from 105 patients with CF were collected in 2011–2012. The isolates were typed by PFGE and screened for susceptibility to antibiotics and presence of resistance and virulence genes. Small colony variants (SCV) were identified and tested for auxotrophy. Results: Total of 142/58 (isolates/patients) were resistant to MLSb antibiotics (Macrolides-Lincosamides-Streptogramin B), 25/13 were resistant to aminoglycosides and 6/5 were MRSA. MLSb resistance was associated with genes from erm family or msrA gene. Isolates resistant to aminoglycosides harboured aadC, aphA3 or aac-aphD gene. mecA gene was detected in all of 6 MRSA isolates. Most frequent virulence genes detected in the isolates (n = 242) were genes for enterotoxins seg (n = 137), sei (n = 128), sec (n = 38), sea (n = 19), sej (n = 15) and seh (n = 18). Other virulence genes were not found or found sporadically. Isolates with SCV phenotype (n = 19), all thymidine auxotrophs, were isolated in 9 patients. Isolates were separated in to the 39 different pulsotypes by PFGE with two dominant pulsotypes of 61 isolates from 27 patient (26%) and 28 isolates from 10 patients (12%) respectively. |
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