Publication details

Vagococcus entomophilus sp. nov., from the digestive tract of a wasp (Vespula vulgaris)

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Authors

KILLER Jiří ŠVEC Pavel SEDLÁČEK Ivo ČERNOHLÁVKOVÁ Jitka BENADA O. HRONCOVÁ Z. HAVLÍK J. VLKOVÁ E. RADA Vojtěch KOPEČNÝ J. KOFROŇOVÁ O.

Year of publication 2014
Type Article in Periodical
Magazine / Source International Journal of Systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
web http://ijs.sgmjournals.org/content/64/Pt_3/731.abstract?sid=662b2592-1193-463a-b12c-9d61b9d45f05
Doi http://dx.doi.org/10.1099/ijs.0.054940-0
Field Microbiology, virology
Keywords Vagococcus entomophilus sp. nov.; Vespula vulgaris; taxonomy
Description Three unknown Gram-stain-positive, catalase-negative, facultatively anaerobic and coccus-shaped strains of bacteria were isolated from the digestive tracts of wasps (Vespula vulgaris). Analysis of 16S rRNA gene sequences revealed that these strains had identical sequences and showed that Vagococcus salmoninarum, with 96.2 % sequence similarity, was the closest phylogenetic neighbour. Further analyses based on hsp60 and pheS gene sequences of representatives of the family Enteroccocaceae and genotypic and phenotypic characterization using (GTG)5-PCR fingerprintings, EcoRI ribotyping, DNA G+C content, whole-cell protein profiling, cellular fatty acid profiles analysis and extensive biotyping confirmed that the investigated strains were representatives of a novel bacterial species within the genus Vagoccocus for which the name Vagoccocus entomophilus sp. nov. is proposed. The type strain is VOSTP2T (=DSM 24756T = CCM 7946T).
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