Publication details
Vliv nanočástic stříbra na vybrané imunitní parametry bezobratlých (Galerie Mellonella)
| Title in English | Influence of silver nanoparticles on selected immune parameter of invertebrate (Galerie Mellonella) |
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| Authors | |
| Year of publication | 2014 |
| Type | Article in Proceedings |
| Conference | Tomáškovy dny 2014 |
| MU Faculty or unit | |
| Citation | |
| Field | Physiology |
| Keywords | nanoparticles; silver; Galerie Mellonella; lysozyme; phenoloxidase; antioxidant capacity |
| Description | Silver nanoparticles in the environment is polluting factor produced by man. Therefore research of properties of this nanoparticle is gaining in importance and popularity. Antimicrobial properties of silver nanoparticles are generally known. In this work we focused on examining the impact of silver nanoparticles on selected immune parameters in wax moth (Galerie Mellonella). Because of the similarities between innate immunity of invertebrates and vertebrates, the result can be used to simulate effects of nanoparticles on human. We used electrolytically produced silver nanoparticles about 100 nm in diameter in our experiments. The moth larvae in VII. instar were affected by the nanoparticles. 7 µl of a solution of nanoparticles at a concentration of 50 ppm was injected them through pseudopod just under the cuticle directly into the hemolymph. The larvae were then incubated for a couple days (2 days , 3 days and 5 days) then the hemolymph was collected . In the larvae that were affected by silver nanoparticles, we measure the activity of phenoloxidase cascade by spectrophotometer. The second parameter was activity of lysozyme measured by radial diffusion. The third selected parameter was antioxidant capacity of hemolymph, it was determined using luminometer by TRAP method (total radical-trapping antioxidant parameter). Value of phenoloxidases activity was reduced compared to the control. The antioxidant capacity was lower too. Also, it is seen that the longer the larvae were exposed to the effect of nanoparticles the more decreased measured parameters was. The activity of lysozyme is also decreasing when the number of days of incubation is increased. The results of these method, indicate that immunity is decreased after exposure to the effects of silver nanoparticles in their body. This leads to an interesting dilemma. On the one hand, the silver nanoparticles are capable of destroying microbial organisms, but on the other hand, can also reduce the immune response of the affected organism. |