Publication details
Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens
| Authors | |
|---|---|
| Year of publication | 2015 |
| Type | Article in Periodical |
| Magazine / Source | Microbial Drug Resistance |
| MU Faculty or unit | |
| Citation | |
| Doi | http://dx.doi.org/10.1089/mdr.2014.0210 |
| Field | Microbiology, virology |
| Keywords | SPECTRUM BETA-LACTAMASES; MULTIPLEX PCR; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; ENTEROBACTERIACEAE; RESISTANCE; GENES; INFECTIONS; SHV |
| Description | The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients’ clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL in 6 hours. The rapid method is necessary for early and adequate antimicrobial treatment. |