Publication details
Generation of Two Modified Mouse Alleles of the Hic1 Tumor Suppressor Gene
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | GENESIS |
| Citation | |
| Doi | http://dx.doi.org/10.1002/dvg.20719 |
| Keywords | hypermethylated in cancer 1; Hic1 tumor suppressor; gene targeting; a conditional null allele; citrine reporter mice; placenta |
| Description | HIC1 (hypermethylated in cancer 1) is a tumor suppressor gene located on chromosome 17p13.3, a region frequently hypermethylated or deleted in human neoplasias. In mouse, Hic1 is essential for embryonic development and exerts an antitumor role in adult animals. Since Hic1-deficient mice die perinatally, we generated a conditional Hic1 null allele by flanking the Hic1-coding region by loxP sites. When crossed to animals expressing Cre recombinase in a cell-specific manner, the Hic1 conditional mice will provide new insights into the function of Hic1 in developing and mature tissues. Additionally, we used gene targeting to replace sequence-encoding amino acids 186-893 of Hic1 by citrine fluorescent protein cDNA. We demonstrate that the distribution of Hic1-citrine fusion polypeptide corresponds to the expression pattern of wild-type Hic1. Consequently, Hic1-citrine "reporter'' mice can be used to monitor the activity of the Hic1 locus using citrine fluorescence. genesis 49:142-151, 2011. (C) 2011 Wiley-Liss, Inc. |