Publication details

Capillary electrophoresis with mass spectrometry detection for beta-secretase enzyme assay

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Authors

SCHEJBAL Jan SLEZÁČKOVÁ Lucie ŠEFRANÁ Šárka LANGMAJEROVÁ Monika ŘEMÍNEK Roman GLATZ Zdeněk

Year of publication 2015
Type Conference abstract
Citation
Description In this work we present an optimized method for peptide separation and quantitation by capillary electrophoresis with mass spectrometry detection (CE-MS), which can be used for enzyme kinetics and inhibition assays of ß-secretase (BACE). It is a key enzyme involved in creation of amyloid senile plaques, which has been proposed to play essential role in pathogenesis of the Alzheimer’s disease. As a result the ongoing medical and pharmacological research is thus focused on the prevention of the production of beta-amyloid by inhibiting of BACE. The Fluorescence Resonance Energy Transfer based assays are used almost exclusively for this purpose, unfortunately these suffer from number of conceptual disadvantages. Employing CE-MS enables high sensitivity, low sample consumption and rapid analysis time, what is more no expensive labelled substrates are necessary.
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