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Nové směry ve studiu biomolekulárních interakcí pomocí kapilární elektroforézy
Title in English | New Directions in the Study of Biomolecular Interactions by Capillary Electrophoresis |
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Authors | |
Year of publication | 2016 |
Type | Article in Periodical |
Magazine / Source | Chemické listy |
MU Faculty or unit | |
Citation | |
Web | http://www.chemicke-listy.cz/docs/full/2016_04_249-257.pdf |
Field | Biochemistry |
Keywords | capillary electrophoresis; biomolecular interactions; frontal analysis; affinity capillary electrophoresis; Hummel Dreyer methods; vacancy peak; vacancy affinity capillary electrophoresis |
Description | This review provides overview of electrophoretic techniques used for determination of binding parameters such as binding constant and number of binding sites. Various approaches based on CE are described. It focuses on the experimental arrangement of methods and on possible results obtained by them. Beside the advantages of CE for determination of binding parameters are emphasized and compare as well. |
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