Publication details

All-in-one detector of circulating mRNA based on a smartphone

Authors

CMIEL Vratislav GUMULEC Jaromír SVOBODA Ondrej RAUDENSKÁ Martina HUDCOVÁ Kristýna SEKORA Jiri BALOGH Jaroslav MASAŘÍK Michal PROVAZNIK Ivo

Year of publication 2016
Type Article in Proceedings
Conference Proceedings of SPIE : Optical Diagnostics and Sensing XVI - Toward Point-of-Care Diagnostics
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.1117/12.2213253
Field Other specializations of internal medicine
Keywords mRNA; smartphone; PCR; cancer; metallothionein
Description Metallothionein is significantly elevated in various tumors, notably in prostate cancer on both mRNA and protein level. We demonstrated a strong predictive potential of free circulating metallothionein 2A isoform mRNA for patients with this cancer. Circulating mRNA detection relies on expensive equipment and requires high level of expertise. In this work we developed compact "all-in-one" laboratory system which replace microvolume spectrophotometer, thermocycler and realtime PCR machines. We managed to design and construct a microprocessor controlled heating/cooling chamber that ensures required temperature gradient. The chamber includes implemented optical system to enable fluorescence excitation and fluorescence analysis using a smart-phone.

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